IntroductionThe genomes, which are approximately 1,800Mb were assembled from a combination of Roche 454 and Sanger genomic sequences. The pisifera genome (P5-build) was assembled from 154.8 million reads from genomic and BAC pool DNA fragment and paired-end linker libraries. This represents ~30-fold raw sequence coverage of the genome. In addition, ~235,000 BAC end reads generated using Sanger sequencing were also included in the P5-build. For the E. oleifera genome (O7-build), 130 million reads (~25-fold raw sequence coverage) from genomic DNA fragment and paired-end linker libraries were used.